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trim33 expression vectors  (Addgene inc)


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    Addgene inc trim33 expression vectors
    Fig. 1 | Deletion of <t>Trim33</t> abates replicative stress. a Immunoblotting analysis of p19/Nras Trim33-WT and Trim33-KO cell lines; n = 3. b Analysis of the RNA-seq data for two Trim33-KO cell lines; n = 3. Venn diagram shows the number of commonly deregulated genes (p adj <0.01, any log2FC) for the two knockout cell lines com- pared to Trim33-WT cells. Top three enriched KEGG pathways are shown (analyzed by Enrichr70). c DNA fiber assays in Trim33-WT and Trim33-KO cells expressing Myc or a Ctrl vector; n = 2. 100 fibers were measured per sample; scale bar = 1 μm. Sig- nificance was determined using Kruskal–Wallis test and Dunn’s multiple compar- isons. d Immunoblotting analysis in Trim33-WT and Trim33-KO cells expressing Myc or Ctrl vector; n = 3. e DNA fiber assays in Trim33-WT and Trim33-KO cells, unchallenged (Ctrl) or released from a 4-h hydroxyurea (HU) treatment (HU-rel); n = 3. 100 fibers were measured per sample; significance was determined as in c). Scale bar = 1 μm. f EdU incorporation analysis in Ctrl and HU-treated Trim33-WT
    Trim33 Expression Vectors, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trim33 expression vectors/product/Addgene inc
    Average 93 stars, based on 5 article reviews
    trim33 expression vectors - by Bioz Stars, 2026-05
    93/100 stars

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    1) Product Images from "Trim33 masks a non-transcriptional function of E2f4 in replication fork progression."

    Article Title: Trim33 masks a non-transcriptional function of E2f4 in replication fork progression.

    Journal: Nature communications

    doi: 10.1038/s41467-023-40847-0

    Fig. 1 | Deletion of Trim33 abates replicative stress. a Immunoblotting analysis of p19/Nras Trim33-WT and Trim33-KO cell lines; n = 3. b Analysis of the RNA-seq data for two Trim33-KO cell lines; n = 3. Venn diagram shows the number of commonly deregulated genes (p adj <0.01, any log2FC) for the two knockout cell lines com- pared to Trim33-WT cells. Top three enriched KEGG pathways are shown (analyzed by Enrichr70). c DNA fiber assays in Trim33-WT and Trim33-KO cells expressing Myc or a Ctrl vector; n = 2. 100 fibers were measured per sample; scale bar = 1 μm. Sig- nificance was determined using Kruskal–Wallis test and Dunn’s multiple compar- isons. d Immunoblotting analysis in Trim33-WT and Trim33-KO cells expressing Myc or Ctrl vector; n = 3. e DNA fiber assays in Trim33-WT and Trim33-KO cells, unchallenged (Ctrl) or released from a 4-h hydroxyurea (HU) treatment (HU-rel); n = 3. 100 fibers were measured per sample; significance was determined as in c). Scale bar = 1 μm. f EdU incorporation analysis in Ctrl and HU-treated Trim33-WT
    Figure Legend Snippet: Fig. 1 | Deletion of Trim33 abates replicative stress. a Immunoblotting analysis of p19/Nras Trim33-WT and Trim33-KO cell lines; n = 3. b Analysis of the RNA-seq data for two Trim33-KO cell lines; n = 3. Venn diagram shows the number of commonly deregulated genes (p adj <0.01, any log2FC) for the two knockout cell lines com- pared to Trim33-WT cells. Top three enriched KEGG pathways are shown (analyzed by Enrichr70). c DNA fiber assays in Trim33-WT and Trim33-KO cells expressing Myc or a Ctrl vector; n = 2. 100 fibers were measured per sample; scale bar = 1 μm. Sig- nificance was determined using Kruskal–Wallis test and Dunn’s multiple compar- isons. d Immunoblotting analysis in Trim33-WT and Trim33-KO cells expressing Myc or Ctrl vector; n = 3. e DNA fiber assays in Trim33-WT and Trim33-KO cells, unchallenged (Ctrl) or released from a 4-h hydroxyurea (HU) treatment (HU-rel); n = 3. 100 fibers were measured per sample; significance was determined as in c). Scale bar = 1 μm. f EdU incorporation analysis in Ctrl and HU-treated Trim33-WT

    Techniques Used: Western Blot, RNA Sequencing, Knock-Out, Expressing, Plasmid Preparation

    Fig. 3 | Deletion of Trim33 promotes E2f4 interactions with chromatin and with the Recql helicase. a Genome browser tracks of E2f4 ChIP-seq in p19/Nras Trim33- WT and Trim33-KO cells; n = 2. b Quantification of ChIP-seq tag coverage at E2f4 peaks in p19/Nras Trim33-WT and Trim33-KO cells. c Relationship between changes (log2FC) in E2f4 binding (normalized E2f4 ChIP-seq tags) and mRNA expression (log2FC) of the encoded gene for p19/Nras Trim33-KO vs Trim33-WT cells. Each dot is a bin of 5 genes sorted by the change in mRNA levels. Trendlines are derived from linear regression analysis. d LC-MS/MS analysis of E2f4 interactome in p19/Nras Trim33-WT and Trim33-KO cells. Known interaction partners are highlighted in blue. e PLA analysis of Recql-E2f4 association in p19/Nras Trim33-WT and Trim33- KO cells stably expressing HA-tagged E2f4 or vector Ctrl; from left, n = 49,49,50,50
    Figure Legend Snippet: Fig. 3 | Deletion of Trim33 promotes E2f4 interactions with chromatin and with the Recql helicase. a Genome browser tracks of E2f4 ChIP-seq in p19/Nras Trim33- WT and Trim33-KO cells; n = 2. b Quantification of ChIP-seq tag coverage at E2f4 peaks in p19/Nras Trim33-WT and Trim33-KO cells. c Relationship between changes (log2FC) in E2f4 binding (normalized E2f4 ChIP-seq tags) and mRNA expression (log2FC) of the encoded gene for p19/Nras Trim33-KO vs Trim33-WT cells. Each dot is a bin of 5 genes sorted by the change in mRNA levels. Trendlines are derived from linear regression analysis. d LC-MS/MS analysis of E2f4 interactome in p19/Nras Trim33-WT and Trim33-KO cells. Known interaction partners are highlighted in blue. e PLA analysis of Recql-E2f4 association in p19/Nras Trim33-WT and Trim33- KO cells stably expressing HA-tagged E2f4 or vector Ctrl; from left, n = 49,49,50,50

    Techniques Used: ChIP-sequencing, Binding Assay, Expressing, Derivative Assay, Liquid Chromatography with Mass Spectroscopy, Stable Transfection, Plasmid Preparation



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    trim33 expression vectors  (Addgene inc)
    93
    Addgene inc trim33 expression vectors
    Fig. 1 | Deletion of <t>Trim33</t> abates replicative stress. a Immunoblotting analysis of p19/Nras Trim33-WT and Trim33-KO cell lines; n = 3. b Analysis of the RNA-seq data for two Trim33-KO cell lines; n = 3. Venn diagram shows the number of commonly deregulated genes (p adj <0.01, any log2FC) for the two knockout cell lines com- pared to Trim33-WT cells. Top three enriched KEGG pathways are shown (analyzed by Enrichr70). c DNA fiber assays in Trim33-WT and Trim33-KO cells expressing Myc or a Ctrl vector; n = 2. 100 fibers were measured per sample; scale bar = 1 μm. Sig- nificance was determined using Kruskal–Wallis test and Dunn’s multiple compar- isons. d Immunoblotting analysis in Trim33-WT and Trim33-KO cells expressing Myc or Ctrl vector; n = 3. e DNA fiber assays in Trim33-WT and Trim33-KO cells, unchallenged (Ctrl) or released from a 4-h hydroxyurea (HU) treatment (HU-rel); n = 3. 100 fibers were measured per sample; significance was determined as in c). Scale bar = 1 μm. f EdU incorporation analysis in Ctrl and HU-treated Trim33-WT
    Trim33 Expression Vectors, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trim33 expression vectors/product/Addgene inc
    Average 93 stars, based on 1 article reviews
    trim33 expression vectors - by Bioz Stars, 2026-05
    93/100 stars
    		  Buy from Supplier

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    Fig. 1 | Deletion of Trim33 abates replicative stress. a Immunoblotting analysis of p19/Nras Trim33-WT and Trim33-KO cell lines; n = 3. b Analysis of the RNA-seq data for two Trim33-KO cell lines; n = 3. Venn diagram shows the number of commonly deregulated genes (p adj <0.01, any log2FC) for the two knockout cell lines com- pared to Trim33-WT cells. Top three enriched KEGG pathways are shown (analyzed by Enrichr70). c DNA fiber assays in Trim33-WT and Trim33-KO cells expressing Myc or a Ctrl vector; n = 2. 100 fibers were measured per sample; scale bar = 1 μm. Sig- nificance was determined using Kruskal–Wallis test and Dunn’s multiple compar- isons. d Immunoblotting analysis in Trim33-WT and Trim33-KO cells expressing Myc or Ctrl vector; n = 3. e DNA fiber assays in Trim33-WT and Trim33-KO cells, unchallenged (Ctrl) or released from a 4-h hydroxyurea (HU) treatment (HU-rel); n = 3. 100 fibers were measured per sample; significance was determined as in c). Scale bar = 1 μm. f EdU incorporation analysis in Ctrl and HU-treated Trim33-WT

    Journal: Nature communications

    Article Title: Trim33 masks a non-transcriptional function of E2f4 in replication fork progression.

    doi: 10.1038/s41467-023-40847-0

    Figure Lengend Snippet: Fig. 1 | Deletion of Trim33 abates replicative stress. a Immunoblotting analysis of p19/Nras Trim33-WT and Trim33-KO cell lines; n = 3. b Analysis of the RNA-seq data for two Trim33-KO cell lines; n = 3. Venn diagram shows the number of commonly deregulated genes (p adj <0.01, any log2FC) for the two knockout cell lines com- pared to Trim33-WT cells. Top three enriched KEGG pathways are shown (analyzed by Enrichr70). c DNA fiber assays in Trim33-WT and Trim33-KO cells expressing Myc or a Ctrl vector; n = 2. 100 fibers were measured per sample; scale bar = 1 μm. Sig- nificance was determined using Kruskal–Wallis test and Dunn’s multiple compar- isons. d Immunoblotting analysis in Trim33-WT and Trim33-KO cells expressing Myc or Ctrl vector; n = 3. e DNA fiber assays in Trim33-WT and Trim33-KO cells, unchallenged (Ctrl) or released from a 4-h hydroxyurea (HU) treatment (HU-rel); n = 3. 100 fibers were measured per sample; significance was determined as in c). Scale bar = 1 μm. f EdU incorporation analysis in Ctrl and HU-treated Trim33-WT

    Article Snippet: Trim33 expression vectors were a gift of Stefano Piccolo (Addgene plasmids # 20902 and 20903).

    Techniques: Western Blot, RNA Sequencing, Knock-Out, Expressing, Plasmid Preparation

    Fig. 3 | Deletion of Trim33 promotes E2f4 interactions with chromatin and with the Recql helicase. a Genome browser tracks of E2f4 ChIP-seq in p19/Nras Trim33- WT and Trim33-KO cells; n = 2. b Quantification of ChIP-seq tag coverage at E2f4 peaks in p19/Nras Trim33-WT and Trim33-KO cells. c Relationship between changes (log2FC) in E2f4 binding (normalized E2f4 ChIP-seq tags) and mRNA expression (log2FC) of the encoded gene for p19/Nras Trim33-KO vs Trim33-WT cells. Each dot is a bin of 5 genes sorted by the change in mRNA levels. Trendlines are derived from linear regression analysis. d LC-MS/MS analysis of E2f4 interactome in p19/Nras Trim33-WT and Trim33-KO cells. Known interaction partners are highlighted in blue. e PLA analysis of Recql-E2f4 association in p19/Nras Trim33-WT and Trim33- KO cells stably expressing HA-tagged E2f4 or vector Ctrl; from left, n = 49,49,50,50

    Journal: Nature communications

    Article Title: Trim33 masks a non-transcriptional function of E2f4 in replication fork progression.

    doi: 10.1038/s41467-023-40847-0

    Figure Lengend Snippet: Fig. 3 | Deletion of Trim33 promotes E2f4 interactions with chromatin and with the Recql helicase. a Genome browser tracks of E2f4 ChIP-seq in p19/Nras Trim33- WT and Trim33-KO cells; n = 2. b Quantification of ChIP-seq tag coverage at E2f4 peaks in p19/Nras Trim33-WT and Trim33-KO cells. c Relationship between changes (log2FC) in E2f4 binding (normalized E2f4 ChIP-seq tags) and mRNA expression (log2FC) of the encoded gene for p19/Nras Trim33-KO vs Trim33-WT cells. Each dot is a bin of 5 genes sorted by the change in mRNA levels. Trendlines are derived from linear regression analysis. d LC-MS/MS analysis of E2f4 interactome in p19/Nras Trim33-WT and Trim33-KO cells. Known interaction partners are highlighted in blue. e PLA analysis of Recql-E2f4 association in p19/Nras Trim33-WT and Trim33- KO cells stably expressing HA-tagged E2f4 or vector Ctrl; from left, n = 49,49,50,50

    Article Snippet: Trim33 expression vectors were a gift of Stefano Piccolo (Addgene plasmids # 20902 and 20903).

    Techniques: ChIP-sequencing, Binding Assay, Expressing, Derivative Assay, Liquid Chromatography with Mass Spectroscopy, Stable Transfection, Plasmid Preparation